4DIP
Crystal structure of human Peptidyl-prolyl cis-trans isomerase FKBP14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-14 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.120, 64.060, 80.810 |
| Unit cell angles | 81.23, 75.21, 73.38 |
Refinement procedure
| Resolution | 58.950 - 1.820 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.684 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.950 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 101237 | |
| <I/σ(I)> | 8 | 3 |
| Completeness [%] | 96.6 | 95.8 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 277 | 18% PEG_3350, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
