4DIK
Flavo Di-iron protein H90A mutant from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-10 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.219, 96.007, 90.331 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 29.129 - 1.750 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vme |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 94279 | |
| <I/σ(I)> | 24.5 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.1 M sodium acetate, 45% MPD, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
