4DGS
The crystals structure of dehydrogenase from Rhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.657, 71.155, 55.148 |
| Unit cell angles | 90.00, 96.39, 90.00 |
Refinement procedure
| Resolution | 39.581 - 2.500 |
| R-factor | 0.2569 |
| Rwork | 0.255 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dbq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.320 |
| Rmerge | 0.098 | 0.448 |
| Number of reflections | 12548 | |
| <I/σ(I)> | 12.2 | 1.9 |
| Completeness [%] | 95.4 | 69 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Calcium acetate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
