4DGN
Crystal Structure of maize CK2 in complex with the inhibitor luteolin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.863, 59.366, 45.485 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 69.466 - 1.750 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.474 |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.466 | 36.515 | 1.840 |
| High resolution limit [Å] | 1.750 | 5.530 | 1.750 |
| Rmerge | 0.036 | 0.419 | |
| Total number of observations | 2832 | 15299 | |
| Number of reflections | 36948 | ||
| <I/σ(I)> | 10.4 | 24.1 | 2 |
| Completeness [%] | 98.8 | 83.5 | 98.6 |
| Redundancy | 3 | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.1 M Tris-HCl, 10-20% PEG 4000, 0.2 M Na-acetate, pH 8, VAPOR DIFFUSION, temperature 293K |
