4DGK
Crystal structure of Phytoene desaturase CRTI from Pantoea ananatis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791, 0.9794, 0.9770 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.690, 90.690, 130.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.850 - 2.350 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.850 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.056 | 0.353 |
| Number of reflections | 26274 | |
| <I/σ(I)> | 22.5 | 4.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 290 | 8% PEG 8K, 0.1 M NaCl, 0.1 M Na/K phosphate pH 6.2, VAPOR DIFFUSION, temperature 290K |
