4DGF
Structure of SulP Transporter STAS Domain from Wolinella Succinogenes Refined to 1.6 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 37.710, 71.865, 105.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.410 - 1.600 |
| R-factor | 0.20765 |
| Rwork | 0.206 |
| R-free | 0.24384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.177 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 1.201 | |
| Number of reflections | 38860 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 12.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 60% Tacsimate pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
