4DG6
Crystal structure of domains 1 and 2 of LRP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.360, 109.360, 130.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.090 - 2.900 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (1.3.1) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.720 | 32.720 | 2.950 |
| High resolution limit [Å] | 2.880 | 12.860 | 2.880 |
| Rmerge | 0.017 | 0.708 | |
| Number of reflections | 21004 | ||
| <I/σ(I)> | 25 | 35.7 | 1.1 |
| Completeness [%] | 99.6 | 91.2 | 99.9 |
| Redundancy | 7.3 | 5.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 5.9 | 292 | 20% PEG 8000, 20mM ammonium sulfate, pH 5.9, hanging drop, temperature 292K |
