4DFZ
Crystal structure of myristoylated K7C catalytic subunit of cAMP-dependent protein kinase in complex with SP20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.460, 79.630, 118.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.460 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.887 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.460 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30558 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 96.8 | 94.7 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277.15 | Mother liquor: 6% MPD Protein: 50 mM Bicine, 150 mM Ammonium acetate, 10 mM DTT, 8-10 mg/mL protein, 9% MeOH added to well immediately before sealing, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K, pH 8.0 |
