4DFI

Crystal structure of cell adhesion molecule nectin-2/CD112 mutant FAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-01-01
Detector	ENRAF-NONIUS
Wavelength(s)	0.9783
Spacegroup name	P 21 21 21
Unit cell lengths	43.272, 45.780, 52.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.544 - 1.800
R-factor	0.1993
Rwork	0.197
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dfh
RMSD bond length	0.016
RMSD bond angle	1.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	9858
Completeness [%]	96.1	88.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2M ammonium sulfate, 0.1M Tris (pH 8.5), 25% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K