4DF0

Crystal structure of orotidine 5'-monophosphate decarboxylase from Thermoproteus neutrophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	I 41 3 2
Unit cell lengths	171.610, 171.610, 171.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.373 - 1.500
R-factor	0.2067
Rwork	0.205
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.373
High resolution limit [Å]	1.500
Number of reflections	67964
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG 3350, 0.1M Bis-Tris, 0.2M Lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K