4DDV
Thermotoga maritima reverse gyrase, triclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.710, 101.251, 104.427 |
| Unit cell angles | 113.61, 97.50, 110.42 |
Refinement procedure
| Resolution | 49.110 - 3.460 |
| R-factor | 0.2374 |
| Rwork | 0.235 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ddt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS ((VERSION December 6) |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 3.560 |
| High resolution limit [Å] | 3.460 | 3.460 |
| Rmerge | 0.153 | 0.547 |
| Number of reflections | 41027 | |
| <I/σ(I)> | 3.91 | 1 |
| Completeness [%] | 98.3 | 97.5 |
| Redundancy | 1.95 | 1.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M sodium citrate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
