4DDU
Thermotoga maritima reverse gyrase, C2 FORM 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.961, 73.912, 111.371 |
| Unit cell angles | 90.00, 116.59, 90.00 |
Refinement procedure
| Resolution | 44.223 - 3.000 |
| R-factor | 0.2179 |
| Rwork | 0.214 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ddt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.716 |
| Data reduction software | XDS ((VERSION December 6) |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_881)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.274 | 0.941 |
| Number of reflections | 24946 | |
| <I/σ(I)> | 7.09 | 1.11 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 13.2 | 13.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.2M MgCl2, 0.1M HEPES/NaOH pH7.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
