4DCZ

Crystal structure of a domain from a mycoplasma genitalium terminal organelle protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	81.003, 81.003, 73.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.180 - 2.900
R-factor	0.206
Rwork	0.203
R-free	0.26000
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.705
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.067	0.498
Number of reflections	6450
<I/σ(I)>	32.7	5.2
Completeness [%]	74.0	90
Redundancy	10.5	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	22% PEG 2000, 0.1 M SODIUM CITRATE, PH 4.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	22% PEG 2000, 0.1 M SODIUM CITRATE, PH 4.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K