4DBV
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MUTANT WITH LEU 33 REPLACED BY THR, THR 34 REPLACED BY GLY, ASP 36 REPLACED BY GLY, LEU 187 REPLACED BY ALA, AND PRO 188 REPLACED BY SER COMPLEXED WITH NADP+
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | BRUKER NONIUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 134.140, 123.950, 96.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.21900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.890 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.054 * |
| Number of reflections | 52001 |
| Completeness [%] | 92.2 |
| Redundancy | 2.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.9 | pH 6.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2 (M) | |
| 3 | 1 | drop | NAD(P)+ | 30 (mM) | |
| 4 | 1 | drop | phosphate | 50 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.5 (M) |
