4DBQ
MYOSIN VI D179Y (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.310, 107.880, 179.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.975 - 2.600 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.975 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 44526 | |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 50 mM Tris pH 8.5, 10% P8K, 1 mM TCEP, 6% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
