4DBK
Crystal structure of porcine pancreatic phospholipase A2 complexed with berberine
Replaces: 3L69Replaces: 3TT5Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.600, 68.600, 70.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.900 - 2.300 |
| R-factor | 0.18767 |
| Rwork | 0.185 |
| R-free | 0.25313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p2p |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.796 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 7686 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.05M Tris maleate buffer, 5mM CaCl2, 16% MPD, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
