4DB6
Designed Armadillo repeat protein (YIIIM3AII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-10-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.93 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 42.960, 91.580, 92.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.895 - 1.800 |
| R-factor | 0.1837 |
| Rwork | 0.181 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.951 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.570 |
| Rmerge | 0.064 |
| Number of reflections | 26356 |
| <I/σ(I)> | 15.5 |
| Completeness [%] | 98.7 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES,pH 7.5, 1.4 M sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
