4DB5

Crystal structure of Rabbit GITRL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	F 2 3
Unit cell lengths	127.047, 127.047, 127.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.147 - 1.522
R-factor	0.1614
Rwork	0.161
R-free	0.17570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fc0
RMSD bond length	0.007
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.520	4.130	1.520
Rmerge	0.051	0.025	0.688
Number of reflections	26128
<I/σ(I)>	12.5
Completeness [%]	100.0	99.3	100
Redundancy	33.1	32.1	32.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Protein (20 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol; Reservoir (0.1M Bis-Tris Propane pH 7.0, 2.4M DL-Malic acid); Cryoprotection (Reservoir + 30% glycerol), Sitting Drop, Vapor Diffusion, temperature 298K, VAPOR DIFFUSION, SITTING DROP