4DB1
Cardiac human myosin S1dC, beta isoform complexed with Mn-AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.206, 94.310, 110.894 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mys |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (10.2.31) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.010 | 2.600 |
| Rmerge | 0.154 | ||
| Number of reflections | 58495 | ||
| <I/σ(I)> | 9.24 | 23.453 | 2.197 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 5 | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | precipitant: 7.5% PEG 8000, 0.05M MES, 0.05M acetate, 0.25M sodium chloride, 0.01M manganese chloride, 0.1% sodium cholate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
