4DAY
Crystal structure of the RMI core complex with MM2 peptide from FANCM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 75.312, 96.440, 99.025 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.2217 |
| Rwork | 0.216 |
| R-free | 0.32540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mxn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.385 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
| Rmerge | 0.081 | 0.026 | 0.587 |
| Number of reflections | 5711 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.7 | 96.9 | 100 |
| Redundancy | 5.1 | 4.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 298 | 200 mM Na2SO4, 5% polyethylene glycol 3350, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
