4DAU
Structure of 14-3-3 sigma in complex with PADI6 14-3-3 binding motif I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2010-09-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.220, 110.240, 61.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.670 - 2.000 |
| R-factor | 0.1696 |
| Rwork | 0.167 |
| R-free | 0.22770 |
| Starting model (for MR) | 3iqv |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.965 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 20.000 | 2.300 |
| High resolution limit [Å] | 2.000 | 15.000 | 2.000 |
| Rmerge | 0.085 | 0.049 | 0.206 |
| Number of reflections | 18642 | 26 | 6233 |
| <I/σ(I)> | 14.41 | 29.86 | 6.52 |
| Completeness [%] | 97.4 | 86.7 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 26.6% v/v PEG400, 0.19 M calcium chloride, 5% v/v glycerol, 0.095 M HEPES sodium, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
