4DAS
Crystal structure of Bullfrog M ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 210.951, 210.951, 323.002 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.510 - 2.560 |
| R-factor | 0.18867 |
| Rwork | 0.186 |
| R-free | 0.23895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mfr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.692 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.500 | 2.700 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.115 | 0.362 |
| Number of reflections | 259578 | |
| <I/σ(I)> | 5.2 | 1.7 |
| Completeness [%] | 98.0 | 98.5 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 3.0 M sodium formate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
