4DAP
The structure of Escherichia coli SfsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97564076 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 117.728, 117.728, 77.594 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.330 - 2.200 |
| R-factor | 0.20615 |
| Rwork | 0.205 |
| R-free | 0.23079 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.202 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.300 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | 0.510 |
| Number of reflections | 28289 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M sodium cacodylate pH 6.5, 0.8 M tri-sodium citrate, 1 % PEG 6000, 0.01 M Tris-HCl and 0.1 M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
