4DAM

Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95371
Spacegroup name	P 32
Unit cell lengths	150.004, 150.004, 54.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.15515
Rwork	0.153
R-free	0.19199
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eiv
RMSD bond length	0.012
RMSD bond angle	1.523
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.056	0.561
Number of reflections	150872
<I/σ(I)>	42.8	3.5
Completeness [%]	100.0	99.8
Redundancy	11.7	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.2 M diammonium hydrogen citrate, 20% PEG3350, 10% glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K