4D9S

Crystal structure of Arabidopsis thaliana UVR8 (UV Resistance locus 8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 1 21 1
Unit cell lengths	68.117, 51.511, 97.084
Unit cell angles	90.00, 102.89, 90.00

Refinement procedure

Resolution	49.421 - 1.701
R-factor	0.1605
Rwork	0.160
R-free	0.19390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.005
RMSD bond angle	0.986
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	73086
Completeness [%]	99.7	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	MPEG 2K, NACL, pH 6.0, VAPOR DIFFUSION, temperature 293K