4D9K
Crystal structure of Escherichia coli Diaminopropionate ammonia lyase in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-14 |
| Detector | RIGAKU JUPITER 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.778, 94.145, 94.687 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 40.650 - 2.190 |
| R-factor | 0.21574 |
| Rwork | 0.213 |
| R-free | 0.26065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d9i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Rmerge | 0.078 | 0.357 |
| Number of reflections | 71324 | |
| <I/σ(I)> | 18.9 | 2.48 |
| Completeness [%] | 98.3 | 97.5 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 20% PEG 3350, 70mM magnesium chloride, 50mM lithium sulfate, 6mM sodium citrate, 5mM beta mercaptoethanol, 40mM tris HCl pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
