4D9E
D-Cysteine desulfhydrase from Salmonella typhimurium complexed with L-cycloserine (LCS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.420, 165.840, 68.060 |
| Unit cell angles | 90.00, 119.03, 90.00 |
Refinement procedure
| Resolution | 58.080 - 2.470 |
| R-factor | 0.21518 |
| Rwork | 0.213 |
| R-free | 0.26274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d8t |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.510 | 2.570 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.103 | 0.232 |
| Number of reflections | 41742 | |
| <I/σ(I)> | 8.2 | 4.2 |
| Completeness [%] | 87.3 | 58.7 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 1.5M Ammonium sulphate, 15% (v/v) ethylene glycol, 0.1M HEPES, 0.2% Benzamidine, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
