4D94
Crystal Structure of TEP1r
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-11 |
| Detector | SBC-3 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 150.515, 150.515, 226.315 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.866 - 2.700 |
| R-factor | 0.2206 |
| Rwork | 0.219 |
| R-free | 0.24420 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.018 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.880 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | |
| Number of reflections | 71959 | |
| <I/σ(I)> | 28.2 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 294 | 2 M NaH2/K2H PO4, 0.2 M NaCl, 0.1 M imidazole pH 8.0, 50 mM NaK tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
