4D8W
Salmonella typhimurium D-Cysteine desulfhydrase soaked with D-cys shows pyruvate bound 4 A away from active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-01-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.290, 165.190, 68.060 |
| Unit cell angles | 90.00, 118.71, 90.00 |
Refinement procedure
| Resolution | 48.380 - 2.010 |
| R-factor | 0.18478 |
| Rwork | 0.183 |
| R-free | 0.21718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d8t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.097 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.380 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.341 |
| Number of reflections | 85215 | |
| <I/σ(I)> | 15.1 | 3.6 |
| Completeness [%] | 98.2 | 87.9 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 1.5M Ammonium sulphate, 15% (v/v) ethylene glycol, 0.1M HEPES, 0.2% Benzamididne, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
