4D8U
Crystal structure of D-Cysteine desulfhydrase from Salmonella typhimurium at 3.3 A in monoclinic space group with 8 subunits in the asymmetric unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.463, 158.140, 181.925 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 52.040 - 3.300 |
| R-factor | 0.24756 |
| Rwork | 0.246 |
| R-free | 0.27462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d8t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.690 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.660 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.123 | 0.425 |
| Number of reflections | 69703 | |
| <I/σ(I)> | 6.7 | 2 |
| Completeness [%] | 90.7 | 87.9 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch method under oil | 8.2 | 298 | 1.8M Na/K Phosphate pH 8.2, Microbatch method under oil, temperature 298K |
