4D8T
Crystal structure of D-Cysteine desulfhydrase from Salmonella typhimurium at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.483, 165.450, 69.208 |
| Unit cell angles | 90.00, 119.01, 90.00 |
Refinement procedure
| Resolution | 29.190 - 2.280 |
| R-factor | 0.19137 |
| Rwork | 0.189 |
| R-free | 0.24182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j0b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.450 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.069 | 0.201 |
| Number of reflections | 63892 | |
| <I/σ(I)> | 11.8 | 5.1 |
| Completeness [%] | 98.4 | 90 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 1.5M Ammonium sulphate, 15% (v/v) ethylene glycol, 0.1M HEPES, 0.2% Benzamidine, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
