4D8M

Crystal structure of Bacillus thuringiensis Cry5B nematocidal toxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 63
Unit cell lengths	115.528, 115.528, 110.328
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.894 - 2.300
R-factor	0.1749
Rwork	0.173
R-free	0.21790
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.132
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.000	2.340
High resolution limit [Å]	2.300	2.900	2.300
Number of reflections	37218
Completeness [%]	99.9	100	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% PEG 3350, 0.2 M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% PEG 3350, 0.2 M sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K