4D82
Metallosphera sedula Vps4 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 151.187, 151.187, 98.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 130.930 - 3.200 |
| R-factor | 0.22961 |
| Rwork | 0.228 |
| R-free | 0.26846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d81 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.900 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.140 | 0.510 |
| Number of reflections | 21217 | |
| <I/σ(I)> | 9.9 | 3.1 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 1,4 M SODIUM/POTASSIUM PHOSPHATE PH 5.6 (1.26 M SODIUM DIHYDROGEN PHOSPHATE MONOHYDRATE AND 0.14 M DI-POTASSIUM HYDROGEN PHOSPHATE) AND 7.5% (V/V) GLYCEROL |
