4D80
Metallosphera sedula Vps4 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.700, 127.390, 191.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.020 - 3.600 |
| R-factor | 0.26688 |
| Rwork | 0.264 |
| R-free | 0.31822 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.800 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 27368 | |
| <I/σ(I)> | 8.31 | 1.76 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20 MM TRIS, 50 MM NACL, 0.2 M SODIUM MALONATE PH 7, 19% PEG 3350 |
