Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-01 |
| Detector | RAYONIX SX-165mm |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 65.628, 65.628, 178.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.600 - 1.550 |
| R-factor | 0.17816 |
| Rwork | 0.176 |
| R-free | 0.21669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2trt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.051 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 1.080 |
| Number of reflections | 28546 | |
| <I/σ(I)> | 26.5 | 2.94 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 10.8 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 50 MM TRIS-HCL PH 9.0, 1 M (NH4)2SO4, 70 MM NACL, 10 MM MGCL2 |
