4D7E
An unprecedented NADPH domain conformation in Lysine Monooxygenase NbtG from Nocardia farcinica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.640, 121.920, 91.860 |
| Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
| Resolution | 91.190 - 2.400 |
| R-factor | 0.21064 |
| Rwork | 0.208 |
| R-free | 0.28148 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.980 |
| Number of reflections | 68260 | |
| <I/σ(I)> | 12.3 | 1 |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% W/V PEG6000, 5% V/V 2, 4-METHYLPENTANDIOL (MPD), 0.1 M HEPES/NAOH PH 7.5. |
