4D7C
Monoclinic crystal form of the extracellular olfactomedin domain from gliomedin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.550, 141.700, 46.030 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 43.084 - 1.450 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d77 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.029 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.100 | 1.020 |
| Number of reflections | 78451 | |
| <I/σ(I)> | 8.4 | 1.3 |
| Completeness [%] | 98.7 | 97.4 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM SODIUM CITRATE PH 5.5, 20% PEG 3000 |
