4D77
High-resolution structure of the extracellular olfactomedin domain from gliomedin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.840, 62.480, 88.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.683 - 1.481 |
| R-factor | 0.1386 |
| Rwork | 0.137 |
| R-free | 0.16280 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.050 | |
| Number of reflections | 42999 | |
| <I/σ(I)> | 18 | 1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CHES (PH 9.5) CONTAINING 20% PEG8000 |
