4D74
1.57 A crystal structure of erwinia amylovora tyrosine phosphatase amsI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.026, 66.026, 72.273 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.180 - 1.570 |
| R-factor | 0.16934 |
| Rwork | 0.169 |
| R-free | 0.17922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wmy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.591 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.270 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 25949 | |
| <I/σ(I)> | 23.2 | 5.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.7 | 20.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | MICROBATCH UNDER OIL 1UL PROTEIN ADDED TO 1 UL AMMONIUM SULPHATE 1.7 M, 100MM SODIUM ACETATE PH 5.5. |
