4D6X
Crystal structure of the receiver domain of NtrX from Brucella abortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.670, 110.940, 119.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.640 - 2.110 |
| R-factor | 0.2137 |
| Rwork | 0.213 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l5z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.640 | 2.240 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 30141 | |
| <I/σ(I)> | 16.63 | 1.98 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 5.11 | 5.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 27% PEG 550 MME; 0.15 M MALIC ACID, PH 7.0; 0.1 M IMIDAZOLE, PH 6.9. |
