4D6V
Crystal structure of signal transducing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.673, 81.673, 135.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.016 - 2.200 |
| R-factor | 0.1965 |
| Rwork | 0.195 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3frx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | iMOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.930 |
| Number of reflections | 187760 | |
| <I/σ(I)> | 13.5 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM CACODYLATE,1M SODIUM CITRATE, PH 6.5 |
