4D6N
THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 10 DAYS INCUBATION IN 5MM MG (STATE 7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.311, 70.269, 107.169 |
| Unit cell angles | 90.00, 119.59, 90.00 |
Refinement procedure
| Resolution | 46.598 - 2.350 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vs8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 56760 | |
| <I/σ(I)> | 10.6 | 2.3 |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
