Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

4D6J

Crystal structure of a family 98 glycoside hydrolase catalytic module (Sp3GH98) in complex with the type 2 blood group A-tetrasaccharide (E558A X01 mutant)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-002
Temperature [K]100
DetectorRIGAKU R-AXIS 4
Spacegroup nameC 2 2 21
Unit cell lengths92.240, 154.670, 96.770
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.650 - 1.980
R-factor0.16339
Rwork0.161
R-free0.20598
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2wmi
RMSD bond length0.010
RMSD bond angle1.192
Data reduction softwareMOSFLM
Data scaling softwareAimless
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.6502.090
High resolution limit [Å]1.9801.980
Rmerge0.1500.460
Number of reflections48464
<I/σ(I)>7.82.9
Completeness [%]96.799.8
Redundancy4.44.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
115% PEG 3350, 0.2M AMMONIUM SULFATE, 0.1M SODIUM ACETATE PH 4.8

229380

PDB entries from 2024-12-25

PDB statisticsPDBj update infoContact PDBjnumon