4D69

SOYBEAN AGGLUTININ FROM GLYCINE MAX IN COMPLEX WITH THE ANTIGEN Tn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	77.2
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 32
Unit cell lengths	114.232, 114.232, 202.893
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	202.890 - 2.700
R-factor	0.23146
Rwork	0.230
R-free	0.27433
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sbf
RMSD bond length	0.012
RMSD bond angle	1.591
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.120	0.740
Number of reflections	81148
<I/σ(I)>	10.8	2.3
Completeness [%]	99.8	100
Redundancy	4.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1