4D63
Structure of the carboxy-terminal domain of the turkey type 3 siadenovirus fibre, avirulent form complexed with 6-sialyllactose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-03 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 98.460, 98.460, 98.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.17885 |
| Rwork | 0.177 |
| R-free | 0.21477 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.643 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.580 |
| Number of reflections | 7836 | |
| <I/σ(I)> | 18.8 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25MM MORPHOLINO-ETHANESULFONIC ACID, 0.5-1.0 M DIAMMONIUM PHOSPHATE, 0.1M SODIUM CITRATE PH 5.5-5.8, 0.2-0.3M SODIUM CHLORIDE |
