4D5C
tetracycline repressor class J, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2014-10-02 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.150, 106.060, 70.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.030 - 1.700 |
| R-factor | 0.17486 |
| Rwork | 0.174 |
| R-free | 0.19722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.810 |
| Number of reflections | 21903 | |
| <I/σ(I)> | 29.7 | 2 |
| Completeness [%] | 95.7 | 71.1 |
| Redundancy | 8.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.0 M (NH4)2SO4, 0.07 M NACL, 0.05 M HEPES PH 7.0; CRYO 1.0 M (NH4)2SO4, 0.07 M NACL, 0.05 M HEPES PH 7.0, 10% GLYCEROL |
