4D51
Crystal structure of CymA from Klebsiella oxytoca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-24 |
| Detector | DEXTRIS PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.620, 228.120, 60.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.523 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v3g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.071 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.170 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 23823 | |
| <I/σ(I)> | 16.4 | 2.4 |
| Completeness [%] | 98.0 | 87 |
| Redundancy | 6.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 35% PEG400, 0.4 M NABR, 50 MM GLYCINE PH 9 |
