4D4X
Nitrosyl complex of the D121I variant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-20 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 53.155, 53.155, 180.501 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.030 - 1.300 |
R-factor | 0.17095 |
Rwork | 0.169 |
R-free | 0.21444 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.017 |
RMSD bond angle | 2.353 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.400 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.070 | 0.680 |
Number of reflections | 37815 | |
<I/σ(I)> | 15.1 | 1.73 |
Completeness [%] | 98.7 | 98.6 |
Redundancy | 5.9 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | AMMONIUM SULFATE, HEPES PH 7.5 |