4D4R
Focal Adhesion Kinase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-13 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 115.760, 44.811, 118.076 |
| Unit cell angles | 90.00, 106.54, 90.00 |
Refinement procedure
| Resolution | 55.490 - 1.550 |
| R-factor | 0.20232 |
| Rwork | 0.201 |
| R-free | 0.22064 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.148 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.600 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.340 |
| Number of reflections | 84613 | |
| <I/σ(I)> | 7.9 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 8.5, 0.250 M LISO4, 22% PEG4000, 10 MM TCEP |
