4D4N
Nitrosyl complex of the D121A variant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.756, 53.756, 180.023 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.550 - 1.450 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.20074 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.300 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.730 |
| Number of reflections | 27816 | |
| <I/σ(I)> | 12.1 | 2.5 |
| Completeness [%] | 97.5 | 97.1 |
| Redundancy | 5.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULFATE, HEPES PH 7.5 |
